Homing and retention of MM cells in the humanized niche
Thursday 16 April 2009
Project
In order to investigate MM cells from patients, we have developed a new and unique mouse model, in which we create a 3-D natural “niche” for MM cell growth. One of the features observed in this new humanized mouse model is that patient derived myeloma cells, when injected into the blood stream, can actively home to and retain in the subcutaneously created niche. In this project we would like to investigate the mechanisms responsible for these phenomena. We will create viral vectors which enable us to either overexpress or knock down genes, which are involved in the attraction and adhesion, in both the MSCs as well as the MM cells. These cells will then be tested in adhesion and migration assays in vitro, and if successful finally in vivo in our mouse model.
Techniques
Cell culture, FACS analysis/sorting, viral gene marking, molecular imaging (BLI-Fluor) in animal experiments (optional), luciferase assay, liposome production, MSC expansion/differentiation, immuno-histochemistry
Duration
6 or 9 months
Contact
Dr Anton Martens, a.martens@umcutrecht.nl, 088 75 540 09
Dr Kristin Denzer, k.denzer@umcutrecht.nl, 088 75 576 73
More info
UMC Utrecht - dept. of Immunology
back